Hotspot

trnN-GUU-trnR-ACG

IRa · 133727-134462 · 736 bp

Back to Abelia

Pi 0.0277
MarkerSeek score 75.10
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
736
variable_sites
61
pi
0.0277
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 AGAAGTTCCGGGAGTTACGA CCTCGCAATCACTAGCCAAT 50.0 50.0 58.1 58.0 1407-1415 bp 1.0 90.0
trnN-GUU-trnR-ACG_p2 TGGGTTGAGAACGGGAATTG CCTTGTTCCTACATGACCCG 50.0 55.0 58.1 58.0 1528-1536 bp 1.0 89.6
trnN-GUU-trnR-ACG_p3 AGAAGTTCCGGGAGTTACGA CCTTGTTCCTACATGACCCG 50.0 55.0 58.1 58.0 1574-1582 bp 1.0 89.5
trnN-GUU-trnR-ACG_p4 ATACGATAGCGGCTAGTCGA CCTTGTTCCTACATGACCCG 50.0 55.0 57.9 58.0 1970-1978 bp 1.0 88.4
trnN-GUU-trnR-ACG_p5 TACGATAGCGGCTAGTCGAT CCTTGTTCCTACATGACCCG 50.0 55.0 57.9 58.0 1969-1977 bp 1.0 88.4