Hotspot

rps15-ycf1

SSC · 127746-128227 · 482 bp

Back to Abelia

Pi 0.0309
MarkerSeek score 79.10
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
482
variable_sites
69
pi
0.0309
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 GCAGACCCCCTGTTTTCTTT TGGAAGTCGTTTCAGCATGT 50.0 45.0 58.3 57.7 601-622 bp 1.0 87.6
rps15-ycf1_p2 TTGAAATTCAGCAGACCCCC TGGAAGTCGTTTCAGCATGT 50.0 45.0 58.1 57.7 611-632 bp 0.9 85.4
rps15-ycf1_p3 GAAATTCAGCAGACCCCCTG TGGAAGTCGTTTCAGCATGT 55.0 45.0 58.5 57.7 609-630 bp 0.9 81.7
rps15-ycf1_p4 TGAAATTCAGCAGACCCCCT TGGAAGTCGTTTCAGCATGT 50.0 45.0 58.9 57.7 610-631 bp 0.9 78.6
rps15-ycf1_p5 TTGAAATTCAGCAGACCCCC ATGGAAGTCGTTTCAGCATGT 50.0 42.9 58.1 58.2 612-633 bp 0.9 77.7