Hotspot

trnR-ACG-trnN-GUU

IRb · 113226-113961 · 736 bp

Back to Abelia

Pi 0.0283
MarkerSeek score 75.30
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
736
variable_sites
61
pi
0.0283
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CCTCGCAATCACTAGCCAAT AGAAGTTCCGGGAGTTACGA 50.0 50.0 58.0 58.1 1407-1415 bp 1.0 90.0
trnR-ACG-trnN-GUU_p2 TGTCCCCTCCAGTCAAGAAT AGAAGTTCCGGGAGTTACGA 50.0 50.0 58.0 58.1 1432-1440 bp 1.0 89.9
trnR-ACG-trnN-GUU_p3 CCTTGTTCCTACATGACCCG TGGGTTGAGAACGGGAATTG 55.0 50.0 58.0 58.1 1528-1536 bp 1.0 89.6
trnR-ACG-trnN-GUU_p4 CCTTGTTCCTACATGACCCG AGAAGTTCCGGGAGTTACGA 55.0 50.0 58.0 58.1 1574-1582 bp 1.0 89.5
trnR-ACG-trnN-GUU_p5 GTCAAGTGGAAGTGCAGTGA AGAAGTTCCGGGAGTTACGA 50.0 50.0 58.0 58.1 1635-1643 bp 1.0 89.1