Hotspot

GFC95_pgr005-trnR-ACG

IRb · 112891-113151 · 261 bp

Back to Abelia

Pi 0.112
MarkerSeek score 91.30
Primer available yes
Label GFC95_pgr005-trnR-ACG

Feature Metadata

label_name
GFC95_pgr005-trnR-ACG
feature_type
igs
region
IRb
length_bp
261
variable_sites
62
pi
0.112
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
91.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
GFC95_pgr005-trnR-ACG_p1 AAGCTTCACTGTTCCTTGGG AGAAGTTCCGGGAGTTACGA 50.0 50.0 58.0 58.1 2500-2508 bp 1.0 87.2
GFC95_pgr005-trnR-ACG_p2 AAGCTTCACTGTTCCTTGGG TGGGTTGAGAACGGGAATTG 50.0 50.0 58.0 58.1 2454-2462 bp 1.0 87.2
GFC95_pgr005-trnR-ACG_p3 CATGTCTGTGAAGATGCGGA AGAAGTTCCGGGAGTTACGA 50.0 50.0 58.0 58.1 2550-2558 bp 1.0 87.1
GFC95_pgr005-trnR-ACG_p4 ATGTCTGTGAAGATGCGGAC AGAAGTTCCGGGAGTTACGA 50.0 50.0 58.0 58.1 2549-2557 bp 1.0 87.1
GFC95_pgr005-trnR-ACG_p5 TAGCGAAATTCCTTGTCGGG AGAAGTTCCGGGAGTTACGA 50.0 50.0 58.0 58.1 2639-2647 bp 1.0 87.0