Hotspot

petB-petD

LSC · 80529-81447 · 919 bp

Back to Abelia

Pi 0.0125
MarkerSeek score 69.60
Primer available yes
Label petB-petD

Feature Metadata

label_name
petB-petD
feature_type
igs
region
LSC
length_bp
919
variable_sites
29
pi
0.0125
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB-petD_p1 GCCACTTCTTACTGCGGTAT ACAGGATCATTCAGGTCAGG 50.0 50.0 57.7 56.6 1075-1093 bp 1.0 69.3
petB-petD_p2 GCCACTTCTTACTGCGGTAT CAGGATCATTCAGGTCAGGT 50.0 50.0 57.7 56.6 1074-1092 bp 1.0 69.3
petB-petD_p3 GCCACTTCTTACTGCGGTAT ACAGGATCATTCAGGTCAGGT 50.0 47.6 57.7 58.4 1075-1093 bp 1.0 68.8
petB-petD_p4 ATTGCCACTTCTTACTGCGG ACAGGATCATTCAGGTCAGG 50.0 50.0 58.5 56.6 1078-1096 bp 1.0 67.4
petB-petD_p5 ATTGCCACTTCTTACTGCGG CAGGATCATTCAGGTCAGGT 50.0 50.0 58.5 56.6 1077-1095 bp 1.0 67.4