Hotspot

psbJ-psbL

LSC · 68856-69050 · 195 bp

Back to Abelia

Pi 0.0336
MarkerSeek score 76.20
Primer available yes
Label psbJ-psbL

Feature Metadata

label_name
psbJ-psbL
feature_type
igs
region
LSC
length_bp
195
variable_sites
18
pi
0.0336
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbJ-psbL_p1 CCCAATCCGGAATATGACCC GCTACGACACAATCAAACCC 55.0 50.0 57.8 57.1 412-429 bp 1.0 78.1
psbJ-psbL_p2 GGATGAACCCAATCCGGAAT GCTACGACACAATCAAACCC 50.0 50.0 57.6 57.1 419-436 bp 1.0 76.6
psbJ-psbL_p3 CCAATCCGGAATATGACCCA GCTACGACACAATCAAACCC 50.0 50.0 57.1 57.1 411-428 bp 1.0 72.1
psbJ-psbL_p4 ACCCAATCCGGAATATGACC GCTACGACACAATCAAACCC 50.0 50.0 57.0 57.1 413-430 bp 1.0 72.1
psbJ-psbL_p5 CCCAATCCGGAATATGACCC GCTACGACACAATCAAACCCA 55.0 47.6 57.8 58.9 412-429 bp 1.0 70.4