Hotspot

rbcL-accD

LSC · 59268-61104 · 1837 bp

Back to Abelia

Pi 0.052
MarkerSeek score 83.80
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
1837
variable_sites
259
pi
0.052
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AATGAGGGACGTGATCTTGC AGGGAGTGGAATCCTGTTCA 50.0 50.0 58.0 58.0 2359-2748 bp 1.0 91.4
rbcL-accD_p2 AATGAGGGACGTGATCTTGC TGAGGGAGTGGAATCCTGTT 50.0 50.0 58.0 58.0 2361-2750 bp 1.0 91.4
rbcL-accD_p3 AATGAGGGACGTGATCTTGC TGCTTGTATGAGGGAGTGGA 50.0 50.0 58.0 58.0 2369-2758 bp 1.0 91.4
rbcL-accD_p4 CGTAGCTAATCGAGTCGCTC AGGGAGTGGAATCCTGTTCA 55.0 50.0 58.1 58.0 2402-2791 bp 1.0 91.2
rbcL-accD_p5 CGTAGCTAATCGAGTCGCTC TGAGGGAGTGGAATCCTGTT 55.0 50.0 58.1 58.0 2404-2793 bp 1.0 91.2