Hotspot

ndhC-trnV-UAC

LSC · 52924-53989 · 1066 bp

Back to Abelia

Pi 0.0131
MarkerSeek score 70.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1066
variable_sites
39
pi
0.0131
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGGGGCTAAAACTCCAGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1179-1221 bp 1.0 81.8
ndhC-trnV-UAC_p2 ATCGGGGCTAAAACTCCAGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1181-1223 bp 1.0 79.9
ndhC-trnV-UAC_p3 AATCGGGGCTAAAACTCCAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 1182-1224 bp 1.0 79.9
ndhC-trnV-UAC_p4 CGGGGCTAAAACTCCAGAAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.8 57.5 1178-1220 bp 1.0 79.0
ndhC-trnV-UAC_p5 TTCCGGCCCTTTACTAATCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.4 57.8 1197-1239 bp 1.0 78.4