Hotspot

psaA-ycf3

LSC · 44127-44944 · 818 bp

Back to Abelia

Pi 0.0181
MarkerSeek score 76.40
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
818
variable_sites
39
pi
0.0181
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 CCAGGTTTAGCCCATTCCTC ATCAAGCCGCTGAGTATTGG 55.0 50.0 57.9 58.0 987-1010 bp 1.0 86.4
psaA-ycf3_p2 CCAGGTTTAGCCCATTCCTC GGAAACAGGCTATAGCGCTT 55.0 50.0 57.9 58.0 969-992 bp 1.0 86.4
psaA-ycf3_p3 CCAGGTTTAGCCCATTCCTC TTGGTTGAAGATCACCAGGC 55.0 50.0 57.9 58.1 920-943 bp 1.0 86.2
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 978-1001 bp 1.0 86.0
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT GGAAACAGGCTATAGCGCTT 50.0 50.0 57.9 58.0 960-983 bp 1.0 86.0