Hotspot

trnE-UUC-trnT-GGU

LSC · 33348-34195 · 848 bp

Back to Abelia

Pi 0.0257
MarkerSeek score 83.30
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
848
variable_sites
54
pi
0.0257
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AACTTCCTTTCGCAGTACCC GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 961-992 bp 1.0 86.9
trnE-UUC-trnT-GGU_p2 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 916-947 bp 1.0 86.5
trnE-UUC-trnT-GGU_p3 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 895-926 bp 1.0 86.5
trnE-UUC-trnT-GGU_p4 AACTTCCTTTCGCAGTACCC CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 958-989 bp 1.0 85.8
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG CCGATGACTTACGCCTTACC 55.0 55.0 58.3 58.2 913-944 bp 1.0 85.4