Hotspot

trnC-GCA-petN

LSC · 29562-30430 · 869 bp

Back to Abelia

Pi 0.0353
MarkerSeek score 82.20
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
869
variable_sites
70
pi
0.0353
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 898-933 bp 1.0 88.2
trnC-GCA-petN_p2 GTATCAATTTGGCGGCATGG AGCCCAAGCGAGACTTACTA 50.0 50.0 57.9 58.2 950-985 bp 1.0 87.4
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 916-951 bp 1.0 85.9
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG TTAGAGCCCACTTCTTCCCC 50.0 55.0 58.1 58.7 961-996 bp 1.0 83.3
trnC-GCA-petN_p5 GTATCAATTTGGCGGCATGG TTAGAGCCCACTTCTTCCCC 50.0 55.0 57.9 58.7 1013-1048 bp 1.0 82.6